vitamin d3 and its analogues Search Results


ro 23–7553  (BioXell Inc)
90
BioXell Inc ro 23–7553
Ro 23–7553, supplied by BioXell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caltrate 600 + d  (Ayerst Laboratories)
90
Ayerst Laboratories caltrate 600 + d
Caltrate 600 + D, supplied by Ayerst Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c_12060045_20  (Thermo Fisher)
98
Thermo Fisher c_12060045_20
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gene exp vdr hs01045844 m1  (Thermo Fisher)
90
Thermo Fisher gene exp vdr hs01045844 m1
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Gene Exp Vdr Hs01045844 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse rat 25 oh d3 elisa kit  (Eagle Biosciences)
93
Eagle Biosciences mouse rat 25 oh d3 elisa kit
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Mouse Rat 25 Oh D3 Elisa Kit, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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vitamin d3  (Carlson Laboratories Inc)
90
Carlson Laboratories Inc vitamin d3
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Vitamin D3, supplied by Carlson Laboratories Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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pecutrin ® mineral supplement plus vitamins a, d3, and e  (Bayer HealthCare Pharmaceuticals Inc)
90
Bayer HealthCare Pharmaceuticals Inc pecutrin ® mineral supplement plus vitamins a, d3, and e
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Pecutrin ® Mineral Supplement Plus Vitamins A, D3, And E, supplied by Bayer HealthCare Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitamin d3 supplementation  (Gilead Sciences)
99
Gilead Sciences vitamin d3 supplementation
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Vitamin D3 Supplementation, supplied by Gilead Sciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitamin+d3+and+its+analogues/VEKLURY/pm32864756-74-317-327
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calcitriol  (Mayo Medical Laboratories)
90
Mayo Medical Laboratories calcitriol
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Calcitriol, supplied by Mayo Medical Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitamin+d3+and+its+analogues/calcitriol/pmc06287393-95-25-26
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vitamin d3 chewable tablets batch no. aha 13303  (IMEDCO America)
90
IMEDCO America vitamin d3 chewable tablets batch no. aha 13303
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Vitamin D3 Chewable Tablets Batch No. Aha 13303, supplied by IMEDCO America, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp vdr hs00172113 m1  (Thermo Fisher)
97
Thermo Fisher gene exp vdr hs00172113 m1
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Gene Exp Vdr Hs00172113 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cyp24a1 hs00167999 m1  (Thermo Fisher)
99
Thermo Fisher gene exp cyp24a1 hs00167999 m1
Expression of CYP27B1, <t>VDR</t> and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.
Gene Exp Cyp24a1 Hs00167999 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of CYP27B1, VDR and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.

Journal: PLoS ONE

Article Title: Vitamin D Induction of the Human Antimicrobial Peptide Cathelicidin in the Urinary Bladder

doi: 10.1371/journal.pone.0015580

Figure Lengend Snippet: Expression of CYP27B1, VDR and CYP24A1 was detected on mRNA level in normal bladder cells (HBEP) and three bladder cells lines (TERT-NHUC, 5637 and T24) by RT-PCR ( A , C , E ) and on protein level in tissue from bladder biopsies by fluorescence immunohistochemistry ( B , D , F ). mRNA expression results were normalized to expression in the kidney epithelial cell line A498. The proteins are stained with AlexaFluor 594 (red), the cell nucleus with DAPI (blue). CYP27B1 protein was detected in the bladder mucosa where it was evenly distributed in the bladder epithelial layers with an abundance of staining seen in the peri-nuclear area, consistent with its association to mitochondria ( B ). VDR protein was localized in the cytoplasm and nucleus ( D ). Low levels of the CYP24A1 protein were detected in bladder mucosa ( F ). All images are captured at ×63 magnification and the right panels of images B, D and F show controls stained without primary antibodies against CYP27B1, VDR and CYP24A1, respectively.

Article Snippet: Real-time PCR was performed on a RotorGene 3000 (Corbett Research) with primers and probes from Applied Biosystems; CAMP Hs00189038_m1, CYP24A1 Hs00167999_m1, CYP27B1 Hs00168017_m1, VDR Hs01045844_m1.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Fluorescence, Immunohistochemistry, Staining

Normal bladder cells (HBEP, upper panel) and TERT-NHUC bladder cells (lower panel) were stimulated with 10 −7 M 25D 3 or 10 −8 M 1,25D 3 for 24 hours. Levels of gene-specific mRNA for CYP24A1 ( A , B ), CAMP ( C , D ), VDR ( E , F ) and CYP27B1 ( G , H ) in stimulated cells were determined and are presented as fold change compared to vector-treated control cells. Data presented are pooled from three independent experiments and shown as mean and standard deviation; *** P <0.001, ** P <0.01, * P <0.05 (ANOVA with Dunnett's post-test).

Journal: PLoS ONE

Article Title: Vitamin D Induction of the Human Antimicrobial Peptide Cathelicidin in the Urinary Bladder

doi: 10.1371/journal.pone.0015580

Figure Lengend Snippet: Normal bladder cells (HBEP, upper panel) and TERT-NHUC bladder cells (lower panel) were stimulated with 10 −7 M 25D 3 or 10 −8 M 1,25D 3 for 24 hours. Levels of gene-specific mRNA for CYP24A1 ( A , B ), CAMP ( C , D ), VDR ( E , F ) and CYP27B1 ( G , H ) in stimulated cells were determined and are presented as fold change compared to vector-treated control cells. Data presented are pooled from three independent experiments and shown as mean and standard deviation; *** P <0.001, ** P <0.01, * P <0.05 (ANOVA with Dunnett's post-test).

Article Snippet: Real-time PCR was performed on a RotorGene 3000 (Corbett Research) with primers and probes from Applied Biosystems; CAMP Hs00189038_m1, CYP24A1 Hs00167999_m1, CYP27B1 Hs00168017_m1, VDR Hs01045844_m1.

Techniques: Plasmid Preparation, Control, Standard Deviation